ICSI Treatment at Orion IVF Pune
Nurturing Dreams, Creating Families with Care and Compassion
ICSI - Advanced Solution for Male Infertility
At Orion IVF Pune, we offer advanced ICSI (Intracytoplasmic Sperm Injection) treatment to effectively address male infertility issues. This cutting-edge procedure involves injecting a single healthy sperm directly into an egg, significantly increasing the chances of fertilization — especially in cases of low sperm count, poor motility, or abnormal morphology.
We recognize that this journey requires both clinical expertise and emotional support. At Orion IVF, our state-of-the-art laboratory and a compassionate team work together to maximize your chances of success. We are committed to turning your hopes into a happy reality. If male infertility has been a challenge for you, we invite you to consult with Dr. Hitnalikar to explore how ICSI can be a vital step toward building your family.
What is ICSI & how is it Different from IVF?
Intracytoplasmic Sperm Injection (ICSI) is an advanced assisted reproductive technology (ART) used as part of the IVF process to address severe male infertility or other fertilization challenges. Unlike conventional IVF, where sperm and egg are combined in a lab dish, ICSI involves the direct injection of a single, healthy sperm into a mature egg to facilitate fertilization. This precise technique significantly improves the chances of successful conception for couples facing specific fertility hurdles.
At Orion IVF, our ICSI treatment is tailored to each patient’s unique needs, ensuring optimal outcomes with a focus on safety and precision.

When Should You Consider ICSI Treatment?
ICSI is primarily recommended for couples experiencing male-factor infertility. However, its application has expanded, and it may be beneficial in various scenarios:
- Low Sperm Count (Oligospermia): When there are very few sperm available.
- Poor Sperm Motility (Asthenozoospermia): When sperm have difficulty moving effectively.
- Abnormal Sperm Morphology (Teratozoospermia): When sperms have an unusual shape, hindering natural fertilization.
- Obstructive Azoospermia: When sperm are produced but cannot be ejaculated due to blockages (e.g., after a vasectomy or congenital absence of vas deferens). In such cases, sperm may be surgically retrieved via procedures like PESA (Percutaneous Epididymal Sperm Aspiration) or TESA (Testicular Sperm Aspiration).
- Previous Failed Fertilization: For couples who have experienced poor or no fertilization in a prior conventional IVF cycle.
- Using Frozen Sperm or Eggs: When using cryopreserved sperm (especially if quality is compromised) or previously frozen eggs, as freezing can sometimes harden the egg’s outer layer.
- Preimplantation Genetic Testing (PGT): To ensure that only the intended sperm fertilizes the egg, preventing contamination from other sperm that could interfere with genetic analysis.
Why Orion IVF is the Best Choice You Can Trust
10,000+
Healthy Babies with IVF Treatment
74% ICSI
Success Rate with Advanced Technology
15+ Years
of IVF & Fertility Expertise
100% Expert
Personalized IVF Treatment
How is the ICSI Process performed at Orion IVF Pune
Our IVF center in Wakad, Pune, follows a meticulous ICSI process to maximize success:
Ovarian Stimulation
The female partner receives hormonal injections for 8-14 days to produce multiple eggs, monitored via ultrasound and blood tests
Egg Retrieval
Mature eggs are collected using a minimally invasive procedure under sedation for comfort.
Sperm Collection and Fertilization
Using micromanipulation tools and high-powered microscopes, our embryologist, Tejal Lingayat, selects a single healthy sperm and injects it into the egg’s cytoplasm.
Embryo Culture
Fertilized eggs are cultured in specialized incubators for 3-5 days, monitored for healthy development
Embryo Transfer
The healthiest embryo is transferred to the uterus using a fine catheter, guided by ultrasound. Excess embryos can be frozen for future use.
Leading the Way in ICSI Treatment – Why Orion IVF Pune Stands Out
As a leading IVF clinic in Pune, Orion IVF offers unparalleled expertise and technology for ICSI treatment in Pune:
- High Fertilization Rates: ICSI fertilizes 50-80% of injected eggs, significantly improving chances of embryo development at our IVF center in Pune.
- Expert Team: Dr. Parag Hitnalikar and Tejal Lingayat, with over 31 years of combined experience, ensure precision and personalized care.
- Advanced Laboratory: Equipped with micromanipulators, time-lapse incubators, and vitrification systems, our lab meets international standards.
- Affordable ICSI Solutions: We provide cost-effective male infertility treatment in Pune, ensuring accessibility without compromising quality. Contact us for pricing details.
- Comprehensive Care: From counseling to post-transfer support, we guide you through every step of ICSI treatment in Pune.
- Tailored for Male Infertility: ICSI addresses severe cases like azoospermia, vasectomy, or poor sperm quality, offering hope where traditional IVF may fail
See the Smiles. Feel the Hope. Trust Orion IVF

We’re finally holding our baby boy after 9 years of waiting
Mr. & Mrs. Kulkarni, Pune
A journey filled with heartbreak finally ended in joy. This couple from Pune is now celebrating life as proud parents, thanks to the expertise and care at Orion IVF Centre

From repeated failures to a miracle baby — Orion gave us hope
Mrs. & Mr. Pawar, Solapur
After multiple failed attempts elsewhere, this couple found success in their very first IVF cycle at Orion. They call their baby girl their “Orion Miracle.”

We had almost given up... until Orion gave us a reason to believe again
Mr. & Mrs. Pawar, Nashik
Dealing with male infertility challenges, the couple embraced ICSI at Orion IVF and are now parents to twins. Their joy is beyond words.

One healthy embryo. One perfect result
Mrs. & Mr. Deshmukh, Kolhapur
After a carefully planned blastocyst transfer, this couple welcomed a healthy baby girl. They say Orion’s lab team and doctors were “the reason we never lost hope.”

Being a mother at 40 felt impossible—until Orion made it real.
Mrs. & Mr. Joshi, Ahmednagar
Struggling with age-related fertility issues, this couple opted for donor egg IVF and successfully conceived. Their story is one of courage, care, and clinical excellence.

After 3 miscarriages, we finally found our rainbow baby at Orion
Mr. & Mrs. Khandwe, Satara
With personalized IVF planning and advanced genetic testing, this couple now has a healthy baby boy. They call the journey “life-changing.”

We were anxious, scared, and broken—but Orion gave us family
Mrs. & Mr. Verma, Pune
This young couple faced unexplained infertility. Through gentle IUI cycles and emotional support, they conceived naturally with Orion’s help.
Our Success Stories

- Google Reviews (4.5/5)
- 200+ Reviews

We’re truly grateful to Dr. Parag for guiding us through our toughest phase. His support, patience, and expert care helped us make the right decisions when we felt lost. Thanks to his thoughtful approach, our dream of parenthood came true. Highly recommend Orion IVF to anyone starting their fertility journey
Ashwini Satpute
Aundh

One of the best IVF centres in wakad pune. Doctor is very soft spoken n friendly. They have a very transparent pricing policy with no hidden costs and the IVF results are also very good. The best centre. Highly recommended.
Geetika Madame
Baramati

Our wholehearted thanks to Dr Parag sir because of whom we became parents of a healthy baby boy. Orion hospital is a great hospital with well experienced doctors and helpful staff. Highly recommend Dr Parag sir’s consultation & treatment to those who are planning for pregnancy. He is very supportive and Soft spoken and one can trust him blindly.
Nidhi Kale
Baner

We are very grateful for the best IVF treatment given to my wife, by Dr Parag and his team. He is very knowledgeable, calm and composed. We had great experience during our pregnancy. The way he treats his patients is commendable, such a positive human. He always makes you feel safe and secure and never recommends you unnecessary tests or medications.
Mukesh Rathod
Hinjewadi

It took us 8 long years to meet our little munchkin.
Dr.Parag is the person who took only 14-15 months to handover the most precious gem of our life. Dr.Parag showed us a ray of hope.He is very positive and expert person in his field.He is very kind,soft spoken and supportive personality. A very big thank you for making it possible to Dr.Parag Hitnalikar and entire Orion family.
Ayesha Mujawar
Wakad

I have delivered a baby boy under the treatment of Dr Parag sir.I think words are not enough to say Thankyou to Dr Parag sir for making our much awaited Dream come true. Dr Parag sir is a very Experienced, Sincere, Dedicated,calm and very knowledgeable doctor who understand the patient problem very well and suggest treatment according to it.
Anju Verma
Baner

We’re truly grateful to Dr. Parag for guiding us through our toughest phase. His support, patience, and expert care helped us make the right decisions when we felt lost. Thanks to his thoughtful approach, our dream of parenthood came true. Highly recommend Orion IVF to anyone starting their fertility journey
Ashwini Satpute
Aundh

One of the best IVF centres in wakad pune. Doctor is very soft spoken n friendly. They have a very transparent pricing policy with no hidden costs and the IVF results are also very good. The best centre. Highly recommended.
Geetika Madame
Baramati

Our wholehearted thanks to Dr Parag sir because of whom we became parents of a healthy baby boy. Orion hospital is a great hospital with well experienced doctors and helpful staff. Highly recommend Dr Parag sir’s consultation & treatment to those who are planning for pregnancy. He is very supportive and Soft spoken and one can trust him blindly.
Nidhi Kale
Baner

We’re truly grateful to Dr. Parag for guiding us through our toughest phase. His support, patience, and expert care helped us make the right decisions when we felt lost. Thanks to his thoughtful approach, our dream of parenthood came true. Highly recommend Orion IVF to anyone starting their fertility journey
Ashwini Satpute
Aundh

One of the best IVF centres in wakad pune. Doctor is very soft spoken n friendly. They have a very transparent pricing policy with no hidden costs and the IVF results are also very good. The best centre. Highly recommended.
Geetika Madame
Baramati

Our wholehearted thanks to Dr Parag sir because of whom we became parents of a healthy baby boy. Orion hospital is a great hospital with well experienced doctors and helpful staff. Highly recommend Dr Parag sir’s consultation & treatment to those who are planning for pregnancy. He is very supportive and Soft spoken and one can trust him blindly.
Nidhi Kale
Baner

We’re truly grateful to Dr. Parag for guiding us through our toughest phase. His support, patience, and expert care helped us make the right decisions when we felt lost. Thanks to his thoughtful approach, our dream of parenthood came true. Highly recommend Orion IVF to anyone starting their fertility journey
Ashwini Satpute
Aundh

One of the best IVF centres in wakad pune. Doctor is very soft spoken n friendly. They have a very transparent pricing policy with no hidden costs and the IVF results are also very good. The best centre. Highly recommended.
Geetika Madame
Baramati

Our wholehearted thanks to Dr Parag sir because of whom we became parents of a healthy baby boy. Orion hospital is a great hospital with well experienced doctors and helpful staff. Highly recommend Dr Parag sir’s consultation & treatment to those who are planning for pregnancy. He is very supportive and Soft spoken and one can trust him blindly.
Nidhi Kale
Baner

We’re truly grateful to Dr. Parag for guiding us through our toughest phase. His support, patience, and expert care helped us make the right decisions when we felt lost. Thanks to his thoughtful approach, our dream of parenthood came true. Highly recommend Orion IVF to anyone starting their fertility journey
Ashwini Satpute
Aundh

One of the best IVF centres in wakad pune. Doctor is very soft spoken n friendly. They have a very transparent pricing policy with no hidden costs and the IVF results are also very good. The best centre. Highly recommended.
Geetika Madame
Baramati

It took us 8 long years to meet our little munchkin.
Dr.Parag is the person who took only 14-15 months to handover the most precious gem of our life. Dr.Parag showed us a ray of hope.He is very positive and expert person in his field.He is very kind,soft spoken and supportive personality. A very big thank you for making it possible to Dr.Parag Hitnalikar and entire Orion family.
Ayesha Mujawar
Wakad
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Most Trusted IVF Clinic in Pune
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Frequently Asked Questions
What is ICSI (Intracytoplasmic Sperm Injection)?
ICSI is an advanced fertility treatment where a single sperm is directly injected into a mature egg in the laboratory to achieve fertilization, as part of an IVF cycle.
When is ICSI treatment recommended?
ICSI is advised for low sperm count, poor motility, previous failed IVF, or when using frozen sperm or surgically retrieved sperm.
How does ICSI differ from traditional IVF?
Unlike traditional IVF, where sperm fertilizes the egg naturally, ICSI involves injecting a single sperm directly into the egg, making it effective for male infertility treatment.
Does ICSI involve surgical sperm retrieval?
Yes, for cases like azoospermia or vasectomy, we use techniques like testicular sperm extraction (TESE) at our IVF center in Pune to retrieve sperm for ICSI.
What is the success rate of ICSI at Orion IVF Pune?
We have a high success rate with ICSI, especially when combined with good embryo quality and advanced lab support. Outcomes depend on individual fertility factors.
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